Coding

Part:BBa_K4623006:Design

Designed by: Ruixiao Tan   Group: iGEM23_BNU-China   (2023-10-09)


Cut Silinker 1(mSA-linker), TrxA-His-thrombin-mSA-GP41-1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 445
    Illegal AgeI site found at 505
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 909


Design Notes

The sequence includes the stop codon


Source

This part sequence is primarily derived from an artificially modified amino acid sequence that has been optimized based on E. coli codons.

References